Isolation of Monomeric Human VHS by a Phage Selection

Isolation and characterization of a phage active against streptomyces griseus

Isolation of human tumor-specific antibodies by selection of an antibody phage library on melanoma cells.

A human single-chain Fv (scFv) library as fusion to phage was constructed from donors with a high titer of autoantibodies. The library was subjected to three rounds of positive selection on human melanoma cells and negative selection on human peripheral blood mononuclear cells. Two scFv clones, B3 and B4, were isolated that bound melanoma cells in cell ELISA and fluorescence-activated cell sort...

Phage Isolation and Investigation

A novel bacteriophage, named phage #3, that infects several major strains of Pseudomonas aeruginosa has been discovered. The phage has a lambdoid morphology as seen by electron microscopy. Its nucleic acid is double stranded DNA and is 10-15 kb in length. Phage immunity tests have shown that the phage is temperate. The phage has been used to mediate transduction in the reconstruction of mutants...

Selection for improved subtiligases by phage display.

Engineering enzyme activity has been challenging because of uncertainties in structure-function relationships and difficulties in screening a large number of mutant enzymes. A product capture strategy using phage display is presented here for the selection of improved enzymes from a large library of variants (>10(9) independently derived mutants). Subtiligase, a double mutant of subtilisin BPN'...

Isolation of a colon tumor specific binding peptide using phage display selection.

Colorectal cancer is the third most common malignancy in the United States. Improved detection sensitivity of early colorectal neoplasms would have important clinical applications. Herein, we report on using phage display to generate peptide libraries that detect colon carcinoma tissues. To accomplish this, we employed positive selection of a peptide-bearing phage library on poorly differentiat...